Understand Antibody Application and Signal Details

This article explains the validated application and image signal interpretation for the antibody HPAXXXXXX. It is intended to help researchers understand how to read the fluorescence channels and how the antibody has been validated for use.

Application details

HPAXXXXXX is validated for immunohistochemistry, or IHC, applications only. If you are planning an experiment, use the antibody within its validated application to support reliable and reproducible results.

Signal interpretation in immunofluorescence images

In immunofluorescence images, the green signal corresponds to the antibody and the plasma membrane. The red signal corresponds to tubulin. When the green and red channels overlap, the combined signal appears yellow.

What the colors mean

• Green: antibody signal and plasma membrane
• Red: tubulin
• Yellow: co-localization of green and red signals

The yellow signal indicates co-localization, which means the green and red channels overlap in the same region. This is a visual mix of the two signals rather than a separate channel.

Additional guidance

If you need help selecting an antibody, interpreting validation data, or confirming whether a product is suitable for your assay, contact Support Atlas Antibodies with your experimental details and product name.